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PEG Chemistry 2019-04-01

· PEGylation and conjugation chemistry

· Characterization of proteins



Preliminary Phase

1. Feasibility study.

2. Screening study to optimize pegylation conditions.

3. Pegylation product separation and purification.

4. Pegylation product characterization (method development for degree of pegylation, precise characterization of conjugation site, measurement of un-pegylated protein and free PEG, mass spectrometry of target products etc.).


Large-scale Study Phase


1. Optimize and scale-up of pegylation conditions, product separation and purification.

2. Product structural characterization (molecular weight determination, conjugation site and pegylation degree).

3. Scale-up production of hundreds of miligram to gram levels of target products.

4. Pegylation product characterization (method development for degree of pegylation, precise characterization of conjugation.

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