Oligoethylenimine grafted PEGylated poly(aspartic acid) as a macromolecular contrast agent: properties and in vivo studies
Bin Jiang, Min Liu, Kunchi Zhang, Guangyue Zu, Jingjin Dong, Yi Cao, Lan Zhang, Renjun Pei
Abstract
PEGylated poly(aspartate-g-OEI) was developed as a magnetic resonance imaging probe. The PEG-PBLA block copolymer was prepared by the ring-opening polymerization of β-benzyl-l-aspartate N-carboxy-anhydride (BLA-NCA) initiated by the terminal primary amino group of mPEG-NH2, by grafting with oligoethylenimine (OEI, Mw = 800) and Gd-DTPA. Compared to Gd-DTPA (4.42 mM-1 s-1), PEG-p(Asp-OEI-DTPA-Gd) exhibited much higher T1 relaxivity (19.03 mM-1 s-1), up to 4.3 times higher than Gd-DTPA. No obvious cytotoxicity was observed from the WST assay and H&E analysis, which illustrated that this macromolecular contrast agent (mCA) exhibited excellent biocompatibility. Folic acid (FA) was further labeled onto the mCA to endow the mCA with targeting ability. During in vivo animal studies, the FA labeled MRI probes showed a significant signal intensity enhancement in the tumor during different time intervals and provided a long and efficient window time for MR examination. These results suggest that such mCAs are excellent candidates as magnetic resonance imaging (MRI) probes with high efficiency and safety.
Related products
Abbreviation: MeO-PEG-NH2
Name: alpha-Methoxy-omega-amino poly(ethylene glycol)
For more product information, please contact us at:
US Tel: 1-844-782-5734
US Tel: 1-844-QUAL-PEG
CHN Tel: 400-918-9898
Email: sales@sinopeg.com
