Maleimidophenyl isocyanates as postpolymerization modification agents and their applications in the synthesis of block copolymers Rikito Takashima, Jumpei Kida, Daisuke Aoki, Hideyuki Otsuka First published: 01 August 2019 https://doi.org/10.1002/pola.29450Citations: 8 ABSTRACT The maleimide structure is highly reactive, exemplified by thiol–ene click reactions with thiols and Diels–Alder reactions with furans.  Although postpolymerization modifications and macromolecular conjugations involving maleimide units have been widely studied, mostly due to their selectivity and high reactivity, little has been reported on the one-pot postpolymerization introduction of maleimides in polymer chains.  Herein, we report p-maleimidophenyl isocyanate and its derivatives as modification agents to introduce maleimide moieties by reaction with hydroxy groups into polymer chains.  The high reactivity of the resulting modification agents and of the corresponding maleimide structures once inserted in the polymer chains was examined by studying their reaction kinetics.  Furthermore, these modification agents were successfully applied to the synthesis of macromonomers for graft polymerization and various block copolymers, with, for example, AB-type, star-shaped, and H-shaped architectures. Related products Abbreviation: 4-arm PEG For more product information, please contact us at: US Tel: 1-844-782-5734 US Tel: 1-844-QUAL-PEG CHN Tel: 400-918-9898 Email: sales@sinopeg.com

Soft Matter. 2016 Jan 21;12(3):798-805. doi: 10.1039/c5sm02015h. Epub 2015 Nov 4. Into the polymer brush regime through the "grafting-to" method: densely polymer-grafted rodlike viruses with an unusual nematic liquid crystal behavior Abstract The current work reports an intriguing discovery of how the force exerted on protein complexes like filamentous viruses by the strong interchain repulsion of polymer brushes can induce subtle changes of the constituent subunits at the molecular scale. Such changes transform into the macroscopic rearrangement of the chiral ordering of the rodlike virus in three dimensions. For this, a straightforward "grafting-to" PEGylation method has been developed to densely graft a filamentous virus with poly(ethylene glycol) (PEG). The grafting density is so high that PEG is in the polymer brush regime, resulting in straight and thick rodlike particles with a thin viral backbone. Scission of the densely PEGylated viruses into fragments was observed due to the steric repulsion of the PEG brush, as facilitated by adsorption onto a mica surface. The high grafting density of PEG endows the virus with an isotropic-nematic (I-N) liquid crystal (LC) phase transition that is independent of the ionic strength and the densely PEGylated viruses enter into the nematic LC phase at much lower virus concentrations. Most importantly, while the intact virus and the one grafted with PEG of low grafting density can form a chiral nematic LC phase, the densely PEGylated viruses only form a pure nematic LC phase. This can be traced back to the secondary to tertiary structural change of the major coat protein of the virus, driven by the steric repulsion of the PEG brush. Quantitative parameters characterising the conformation of the grafted PEG derived from the grafting density or the I-N LC transition are elegantly consistent with the theoretical prediction. Related products Abbreviation: mPEG-NHS For more product information, please contact us at: US Tel: 1-844-782-5734 US Tel: 1-844-QUAL-PEG CHN Tel: 400-918-9898 Email: sales@sinopeg.com

Bioact Mater. 2017 May 6;3(1):48-54.  doi: 10.1016/j.bioactmat.2017.04.007.  eCollection 2018 Mar. Inhibition of HeLa cell growth by doxorubicin-loaded and tuftsin-conjugated arginate-PEG microparticles Abstract In order to improve the release pattern of chemotherapy drug and reduce the possibility of drug resistance, poly(ethylene glycol amine) (PEG)-modified alginate microparticles (ALG-PEG MPs) were developed then two different mechanisms were employed to load doxorubicin (Dox): 1) forming Dox/ALG-PEG complex by electrostatic attractions between unsaturated functional groups in Dox and ALG-PEG;  2) forming Dox-ALG-PEG complex through EDC-reaction between the amino and carboxyl groups in Dox and ALG, respectively.  Additionally, tuftsin (TFT), a natural immunomodulation peptide, was conjugated to MPs in order to enhance the efficiency of cellular uptake.  It was found that the Dox-ALG-PEG-TFT MPs exhibited a significantly slower release of Dox than Dox/ALG-PEG-TFT MPs in neutral medium, suggesting the role of covalent bonding in prolonging Dox retention.  Besides, the release of Dox from these MPs was pH-sensitive, and the release rate was observably increased at pH 6.5 compared to the case at pH 7.4.  Compared with Dox/ALG-PEG MPs and Dox-ALG-PEG MPs, their counterparts further conjugated with TFT more efficiently inhibited the growth of HeLa cells over a period of 48 h, implying the effectiveness of TFT in enhancing cellular uptake of MPs.  Over a period of 48 h, Dox-ALG-PEG-TFT MPs inhibited the growth of HeLa cells less efficiently than Dox/ALG-PEG-TFT MPs but the difference was not significant (p > 0.05).  In consideration of the prolonged and sustained release of Dox, Dox-ALG-PEG-TFT MPs possess the advantages for long-term treatment. Keywords: Cellular uptake;  Chemotherapy;  Controlled release;  Tuftsin. Related products Abbreviation: 4-arm-PEG For more product information, please contact us at: US Tel: 1-844-782-5734 US Tel: 1-844-QUAL-PEG CHN Tel: 400-918-9898 Email: sales@sinopeg.com

We are thrilled to announce that the PharmSci 360 2024 will take place from October 20-23, 2024 in Salt Lake City, Utah. Organized by the American Association of Pharmaceutical Scientists (AAPS), which has been a key player in the pharmaceutical sciences since its inception in 1986, this event brings together over 12,000 members from academia, industry, government, and related organizations worldwide. The overarching goal of AAPS is to enhance the capabilities of pharmaceutical scientists in developing products and therapies that improve global health. PharmSci 360 aims to foster greater collaboration between small molecule and biologics scientists, offering attendees an engaging learning experience that focuses on the latest research and business objectives. The conference will highlight contemporary "hot topics" through cross-disciplinary learning experiences. Participants can look forward to presentations of cutting-edge research, validated scientific methods, and showcases of the latest technologies, services, and products. Keynote speakers will include prominent scientists from the FDA, NIH, leading pharmaceutical companies, and academia. We are also excited to share that SINOEPG will be participating in the exhibition at Booth 2620, where we will showcase our latest technological advancements. We invite everyone to stop by and connect with us! Let’s work together to advance the pharmaceutical sciences and make a positive impact on global health!

Happy Mid-Autumn Festival! As we celebrate this special time with family and friends, we want to remind our valued clients that SINOPEG will have staff regularly checking emails during the holiday. If you have any inquiries or needs, feel free to reach out to us at sales@sinopeg.com. Wishing everyone a joyous festival! #MidAutumnFestival #SINOPEG

Happy Teacher's Day! Today, we honor the incredible educators who inspire, nurture, and shape the minds of the future. Thank you for your dedication and passion in making a difference in our lives! #TeachersDay #ThankATeacher

We are pleased to invite you to join us at the CPHI Milan 2024, taking place on October 8-10, 2024, in Milan, Italy. SINOPEG will be showcasing our latest products and technologies, and we are eager to share our innovations and achievements. Event Details: Event Name: CPHI Milan 2024 Date: October 8-10, 2024 Location: Fiera Milano, Italy SINOPEG is committed to providing high-quality products and excellent services. This exhibition presents a fantastic opportunity for us to discuss potential collaborations, share industry insights, and present our innovative solutions. We warmly invite you to visit our booth #6C92, and we look forward to the opportunity to connect with you. Should you need any further information, please feel free to contact us. Thank you, and we hope to see you there!

Nat Commun.  2020 Mar 18;11(1):1435.   doi: 10.1038/s41467-020-14887-9. Fiber reinforced GelMA hydrogel to induce the regeneration of corneal stroma Bin Kong, Yun Chen, Rui Liu, Xi Liu, Changyong Liu, Zengwu Shao, Liming Xiong, Xianning Liu, Wei Sun, Shengli Mi Abstract Regeneration of corneal stroma has always been a challenge due to its sophisticated structure and keratocyte-fibroblast transformation.   In this study, we fabricate grid poly (ε-caprolactone)-poly (ethylene glycol) microfibrous scaffold and infuse the scaffold with gelatin methacrylate (GelMA) hydrogel to obtain a 3 D fiber hydrogel construct;   the fiber spacing is adjusted to fabricate optimal construct that simulates the stromal structure with properties most similar to the native cornea.   The topological structure (3 D fiber hydrogel, 3 D GelMA hydrogel, and 2 D culture dish) and chemical factors (serum, ascorbic acid, insulin, and β-FGF) are examined to study their effects on the differentiation of limbal stromal stem cells to keratocytes or fibroblasts and the phenotype maintenance, in vitro and in vivo tissue regeneration.   The results demonstrate that fiber hydrogel and serum-free media synergize to provide an optimal environment for the maintenance of keratocyte phenotype and the regeneration of damaged corneal stroma. Related products Abbreviation: 4-arm-PEG For more product information, please contact us at: US Tel: 1-844-782-5734 US Tel: 1-844-QUAL-PEG CHN Tel: 400-918-9898 Email: sales@sinopeg.com