News
  • Micelles with a Loose Core Self-Assembled from Coil-g-Rod Graft Copolymers Displaying High Drug Loading Capacity
    Micelles with a Loose Core Self-Assembled from Coil-g-Rod Graft Copolymers Displaying High Drug Loading Capacity February 26,2024.
    First published: 27 May 2020 https://doi.org/10.1002/macp.202000121 Micelles with a Loose Core Self-Assembled from Coil-g-Rod Graft Copolymers Displaying High Drug Loading Capacity Qijing Huang, Zhanwen Xu, Chunhua Cai, Jiaping Lin Abstract High drug loading capacity is one of the critical demands of micellar drug-delivery vehicles;  however, it is a challenging work.  Herein, it is demonstrated that micelles self-assembled from poly(ethylene glycol)-graft-poly(γ-benzyl-l-glutamate) (PEG-g-PBLG) coil-g-rod graft copolymers display high drug-loading capacity for doxorubicin (DOX) model drugs.  As revealed by a combination study of experiments and dissipative particle dynamics simulations, the high drug-loading capacity of the micelles is related to the loose core structure of the micelles.  In these micelles, the hydrophobic PBLG grafts randomly disperse in the micelle core due to their rigid nature and the coil-g-rod topology of the graft copolymers, which results in a loose core of the micelles.  The structure of the graft copolymer, including the length of rod grafts, the length of coil backbone, and the grafting ratio of the rod grafts affecting the arrangement of the rod grafts in the micelle core has influence on the drug-loading capacity of the micelles.  Besides, the strong π–π stacking interaction between graft copolymers and DOX also plays an important part in achieving high drug-loading capacity.  In vitro studies reveal that these drug-loaded micelles show good biocompatibility, and the DOX can be gradually released from the micelles. Related products Abbreviation: PEG-g-NH2 Name: Poly(ethylene glycol) graft amine For more product information, please contact us at: US Tel: 1-844-782-5734 US Tel: 1-844-QUAL-PEG CHN Tel: 400-918-9898 Email: sales@sinopeg.com
    View More
  • SS-mPEG chemical modification of recombinant phospholipase C for enhanced thermal stability and catalytic efficiency
    SS-mPEG chemical modification of recombinant phospholipase C for enhanced thermal stability and catalytic efficiency February 21,2024.
    Int J Biol Macromol. 2018 May:111:1032-1039.  doi: 10.1016/j.ijbiomac.2018.01.134.  Epub 2018 Jan 31. SS-mPEG chemical modification of recombinant phospholipase C for enhanced thermal stability and catalytic efficiency Xian Fang 1, Xueting Wang 1, Guiling Li 2, Jun Zeng 2, Jian Li 2, Jingwen Liu 3 Abstract PEGylation is one of the most promising and extensively studied strategies for improving the properties of proteins as well as enzymic physical and thermal stability.  Phospholipase C, hydrolyzing the phospholipids offers tremendous applications in diverse fields.  However, the poor thermal stability and higher cost of production have restricted its industrial application.  This study focused on improving the stabilization of recombinant PLC by chemical modification with methoxypolyethylene glycol-Succinimidyl Succinate (SS-mPEG, MW 5000).  PLC gene from isolate Bacillus cereus HSL3 was fused with SUMO, a novel small ubiquitin-related modifier expression vector and over expressed in Escherichia coli.  The soluble fraction of SUMO-PLC reached 80% of the total recombinant protein.  The enzyme exhibited maximum catalytic activity at 80 °C and was relatively thermostable at 40-70 °C.  It showed extensive substrate specificity pattern and marked activity toward phosphatidylcholine, which made it a typical non-specific PLC for industrial purpose.  SS-mPEG-PLC complex exhibited an enhanced thermal stability at 70-80 °C and the catalytic efficiency (Kcat/Km) had increased by 3.03 folds compared with free PLC.  CD spectrum of SS-mPEG-PLC indicated a possible enzyme aggregation after chemical modification, which contributed to the higher thermostability of SS-mPEG-PLC.  The increase of antiparallel β sheets in secondary structure also made it more stable than parallel β sheets.  The presence of SS-mPEG chains on the enzyme molecule surface somewhat changed the binding rate of the substrates, leading to a significant improvement in catalytic efficiency.  This study provided an insight into the addition of SS-mPEG for enhancing the industrial applications of phospholipase C at higher temperature. Keywords: Enzymatic properties;  Phospholipase C;  Recombinant expression;  SS-mPEG modification;  Thermal stability and catalytic efficiency. Related products Abbreviation: mPEG-SS Name: Methoxypoly(ethylene glycol) succinimidyl succinate For more product information, please contact us at: US Tel: 1-844-782-5734 US Tel: 1-844-QUAL-PEG CHN Tel: 400-918-9898 Email: sales@sinopeg.com
    View More
  • Codoping Enhanced Radioluminescence of Nanoscintillators for X-ray-Activated Synergistic Cancer Therapy and Prognosis Using Metabolomics
    Codoping Enhanced Radioluminescence of Nanoscintillators for X-ray-Activated Synergistic Cancer Therapy and Prognosis Using Metabolomics February 18,2024.
    ACS Nano.  2019 Sep 24;13(9):10419-10433.   doi: 10.1021/acsnano.9b04213.   Epub 2019 Aug 21. Codoping Enhanced Radioluminescence of Nanoscintillators for X-ray-Activated Synergistic Cancer Therapy and Prognosis Using Metabolomics Farooq Ahmad 1, Xiaoyan Wang 2, Zhao Jiang 1, Xujiang Yu 1, Xinyi Liu 1, Rihua Mao 3, Xiaoyuan Chen 4, Wanwan Li 1 Abstract Radio- and photodynamic therapies are the first line of cancer treatments but suffer from poor light penetration and less radiation accumulation in soft tissues with high radiation toxicity.   Therefore, a multifunctional nanoplatform with diagnosis-assisted synergistic radio- and photodynamic therapy and tools facilitating early prognosis are urgently needed to fight the war against cancer.   Further, integrating cancer therapy with untargeted metabolomic analysis would collectively offer clinical pertinence through facilitating early diagnosis and prognosis.   Here, we enriched scintillation of CeF3 nanoparticles (NPs) through codoping Tb3+ and Gd3+ (CeF3:Gd3+,Tb3+) for viable clinical approach in the treatment of deep-seated tumors.   The codoped CeF3:Gd3+,Tb3+ scintillating theranostic NPs were then coated with mesoporous silica, followed by loading with rose bengal (CGTS-RB) for later computed tomography (CT)- and magnetic resonance image (MRI)-guided X-ray stimulated synergistic radio- and photodynamic therapy (RT+XPDT) using low-dose, one-time X-ray irradiation.   The results corroborated an efficient tumor regression with synergistic RT+XPDT relative to single RT. Global untargeted metabolome shifts highlighted the mechanism behind this efficient tumor regression using RT, and synergistic RT+XPDT treatment is due to the starvation of nonessential amino acids involved in protein and DNA synthesis and energy regulation pathways necessary for growth and progression.   Our study also concluded that tumor and serum metabolites shift during disease progression and regression and serve as robust biomarkers for early assessment of disease state and prognosis.   From our results, we propose that codoping is an effective and extendable technique to other materials for gaining high optical yield and multifunctionality and for use in diagnostic and therapeutic applications.   Critically, the integration of multifunctional theranostic nanomedicines with metabolomics has excellent potential for the discovery of early metabolic biomarkers to aid in better clinical disease diagnosis and prognosis. Keywords: X-ray inducible photodynamic therapy;   codoped nanoscintillators;   imaging;   metabolomics;   radiotherapy. Related products Abbreviation: mPEG-SS Name: Methoxypoly(ethylene glycol) succinimidyl succinate For more product information, please contact us at: US Tel: 1-844-782-5734 US Tel: 1-844-QUAL-PEG CHN Tel: 400-918-9898 Email: sales@sinopeg.com
    View More
  • Novel free-paclitaxel-loaded redox-responsive nanoparticles based on a disulfide-linked poly(ethylene glycol)-drug conjugate for intracellular drug delivery: synthesis, characterization, and antitumor
    Novel free-paclitaxel-loaded redox-responsive nanoparticles based on a disulfide-linked poly(ethylene glycol)-drug conjugate for intracellular drug delivery: synthesis, characterization, and antitumor February 4,2024.
    Mol Pharm. 2014 Oct 6;11(10):3656-70.  doi: 10.1021/mp500399j.  Epub 2014 Sep 24. Novel free-paclitaxel-loaded redox-responsive nanoparticles based on a disulfide-linked poly(ethylene glycol)-drug conjugate for intracellular drug delivery: synthesis, characterization, and antitumor activity in vitro and in vivo Xingxing Chuan 1, Qin Song, Jialiang Lin, Xianhui Chen, Hua Zhang, Wenbing Dai, Bing He, Xueqing Wang, Qiang Zhang Abstract To address the obstacles facing cancer chemotherapeutics, including toxicity, side effects, water insolubility, and lack of tumor selectivity, a novel stimuli-responsive drug-delivery system was developed based on paclitaxel-loaded poly(ethylene glycol)-disulfide-paclitaxel conjugate nanoparticles (PEG-SS-PTX/PTX NPs).  The formulation emphasizes several benefits, including polymer-drug conjugates/prodrugs, self-assembled NPs, high drug content, redox responsiveness, and programmed drug release.  The PTX-loaded, self-assembled NPs, with a uniform size of 103 nm, characterized by DLS, TEM, XRD, DSC, and (1)H NMR, exhibited excellent drug-loading capacity (15.7%) and entrapment efficiency (93.3%).  PEG-SS-PTX/PTX NPs were relatively stable under normal conditions but disassembled quickly under reductive conditions, as indicated by their triggered-aggregation phenomena and drug-release profile in the presence of dithiothreitol (DTT), a reducing agent.  Additionally, by taking advantage of the difference in the drug-release rates between physically loaded and chemically conjugated drugs, a programmed drug-release phenomenon was observed, which was attributed to a higher concentration and longer action time of the drugs.  The influence of PEG-SS-PTX/PTX NPs on in vitro cytotoxicity, cell cycle progression, and cellular apoptosis was determined in the MCF-7 cell line, and the NPs demonstrated a superior anti-proliferative activity associated with PTX-induced cell cycle arrest in G2/M phase and apoptosis compared to their nonresponsive counterparts.  Moreover, the redox-responsive NPs were more efficacious than both free PTX and the non-redox-responsive formulation at equivalent doses of PTX in a breast cancer xenograft mouse model.  This redox-responsive PTX drug delivery system is promising and can be explored for use in effective intracellular drug delivery. Keywords: paclitaxel;  polymeric nanoparticles;  polymer−drug conjugates/prodrugs;  programmed drug release;  redox-response. Related products Abbreviation: mPEG-SH Name: Methoxypoly(ethylene glycol) thiol Abbreviation: mPEG-PA Name: Methoxypoly(ethylene glycol) propionic acid For more product information, please contact us at: US Tel: 1-844-782-5734 US Tel: 1-844-QUAL-PEG CHN Tel: 400-918-9898 Email: sales@sinopeg.com
    View More
  • Optimization of Surface Coating on Small Pd Nanosheets for in Vivo near-Infrared Photothermal Therapy of Tumor
    Optimization of Surface Coating on Small Pd Nanosheets for in Vivo near-Infrared Photothermal Therapy of Tumor February 1,2024.
    ACS Appl Mater Interfaces. 2015 Jul 8;7(26):14369-75.    doi: 10.1021/acsami.5b03106.    Epub 2015 Jun 24. Optimization of Surface Coating on Small Pd Nanosheets for in Vivo near-Infrared Photothermal Therapy of Tumor Saige Shi 1 2, Yizhuan Huang 1, Xiaolan Chen 1, Jian Weng 2, Nanfeng Zheng 1 Abstract Palladium nanosheets with strong near-infrared absorption have been recently demonstrated as promising photothermal agents for photothermal therapy (PTT) of cancers.    However, systematic assessments of their potential risks and impacts to biological systems have not been fully explored yet.    In this work, we carefully investigate how surface coatings affect the in vivo behaviors of small Pd nanosheets (Pd NSs).    Several biocompatible molecules such as carboxymethyl chitosan (CMC), PEG-NH2, PEG-SH, and dihydrolipoic acid-zwitterion (DHLA-ZW) were used to coat Pd NSs.    The blood circulation half-lives, biodistribution, potential toxicity, clearance, and photothermal effect of different surface-coated Pd NSs in mice after intravenous injection were compared.    PEG-SH-coated Pd NSs (Pd-HS-PEG) were found to have ultralong blood circulation half-life and show high uptake in the tumor.    We then carry out the in vivo photothermal therapeutic studies on the Pd-HS-PEG conjugate and revealed its outstanding efficacy in in vivo photothermal therapy of cancers.    Our results highlight the importance of surface coatings to the in vivo behaviors of nanomaterials and can provide guidelines to the future design of Pd NSs bioconjugates for other in vivo applications. Keywords: in vivo behaviors;    nanosheet;    palladium;    photothermal therapy;    surface coating. Related products Abbreviation: mPEG-SH Name: Methoxypoly(ethylene glycol) thiol Abbreviation: mPEG-NH2 Name: Methoxypoly(ethylene glycol) amine For more product information, please contact us at: US Tel: 1-844-782-5734 US Tel: 1-844-QUAL-PEG CHN Tel: 400-918-9898 Email: sales@sinopeg.com
    View More
  • Autophagy inhibition enabled efficient photothermal therapy at a mild temperature
    Autophagy inhibition enabled efficient photothermal therapy at a mild temperature January 29,2024.
    Biomaterials. 2017 Oct:141:116-124.  doi: 10.1016/j.biomaterials.2017.06.030.  Epub 2017 Jun 27. Autophagy inhibition enabled efficient photothermal therapy at a mild temperature Zhengjie Zhou 1, Yang Yan 1, Kewen Hu 1, Yuan Zou 2, Yiwen Li 2, Rui Ma 1, Qiang Zhang 3, Yiyun Cheng 4 Abstract The heterogeneously-distributed hyperthermia in nanomaterial-mediated photothermal therapy commonly results in incomplete tumor eradication and serious damage of health tissue.  Here, we found autophagy was activated in cancer cells underwent photothermal therapy and the inhibition of autophagy significantly enhanced the efficacy of photothermal killing of cancer cells.  A formulation of chloroquine-loaded polydopamine nanoparticles was developed for sensitized photothermal cancer therapy, and the in vitro and in vivo study demonstrated that inhibition of autophagy remarkably augmented the efficacy of photothermal therapy, leading to efficient tumor suppression at a mild temperature.  The regulation of autophagy provides a new route to increase the efficacy of photothermal cancer therapy. Keywords: Autophagy;  Chloroquine;  Photothermal therapy;  Polydopamine nanoparticles;  Sensitization. Related products Abbreviation: mPEG-SH Name: Methoxypoly(ethylene glycol) thiol Abbreviation: H2N-PEG-SH Name: α-Amino-ω-mercapto poly(ethylene glycol) For more product information, please contact us at: US Tel: 1-844-782-5734 US Tel: 1-844-QUAL-PEG CHN Tel: 400-918-9898 Email: sales@sinopeg.com
    View More
  • Supramolecular hybrid hydrogel based on host-guest interaction and its application in drug delivery
    Supramolecular hybrid hydrogel based on host-guest interaction and its application in drug delivery January 24,2024.
    ACS Appl Mater Interfaces. 2014 Nov 26;6(22):19544-51.  doi: 10.1021/am505649q.  Epub 2014 Nov 14. Supramolecular hybrid hydrogel based on host-guest interaction and its application in drug delivery Jing Yu 1, Wei Ha, Jian-nan Sun, Yan-ping Shi Abstract In this work, we developed a simple, novel method for constructing gold nanocomposite supramolecular hybrid hydrogels for drug delivery, in which gold nanocrystals were utilized as building blocks.  First, methoxypoly(ethylene glycol) thiol (mPEG-SH, molecular weight (MW)=5 K) capped gold nanocrystals (nanospheres and nanorods) were prepared via a facile one-step ligand-exchange procedure.  Then, the homogeneous supramolecular hybrid hydrogels were formed, after adding α-cyclodextrin (α-CD) into PEG-modified gold nanocrystal solutions, due to the host-guest inclusion.  Both gold nanoparticles and inclusion complexes formed between α-CD and PEG chain provided the supra-cross-links, which are beneficial to the gelation formation.  The resulting hybrid hydrogels were fully characterized by a combination of techniques including X-ray diffraction, rheology studies, and scanning electron microscopy.  Meanwhile, the hybrid hydrogel systems demonstrated unique reversible gel-sol transition properties at a certain temperature caused by the temperature-responsive reversible supramolecular assembly.  The drug delivery applications of such hybrid hydrogels were further investigated in which doxorubicin was selected as a model drug for in vitro release, cytotoxicity, and intracellular release studies.  We believe that the development of such hybrid hydrogels will provide new and therapeutically useful means for medical applications. Keywords: drug delivery;  gold nanoparticles;  host−guest inclusion;  supramolecular hybrid hydrogel. Related products Abbreviation: mPEG-SH Name: Methoxypoly(ethylene glycol) thiol For more product information, please contact us at: US Tel: 1-844-782-5734 US Tel: 1-844-QUAL-PEG CHN Tel: 400-918-9898 Email: sales@sinopeg.com
    View More
  • Stability enhanced polyelectrolyte-coated gold nanorod-photosensitizer complexes for high/low power density photodynamic therapy
    Stability enhanced polyelectrolyte-coated gold nanorod-photosensitizer complexes for high/low power density photodynamic therapy January 22,2024.
    Biomaterials. 2014 Aug;35(25):7058-67.    doi: 10.1016/j.biomaterials.2014.04.105.    Epub 2014 May 20. Stability enhanced polyelectrolyte-coated gold nanorod-photosensitizer complexes for high/low power density photodynamic therapy Zhenzhi Shi 1, Wenzhi Ren 1, An Gong 1, Xinmei Zhao 1, Yuehong Zou 1, Eric Michael Bratsolias Brown 2, Xiaoyuan Chen 3, Aiguo Wu 4 Abstract Photodynamic therapy (PDT) is a promising treatment modality for cancer and other malignant diseases, however safety and efficacy improvements are required before it reaches its full potential and wider clinical use.    Herein, we investigated a highly efficient and safe photodynamic therapy procedure by developing a high/low power density photodynamic therapy mode (high/low PDT mode) using methoxypoly(ethylene glycol) thiol (mPEG-SH) modified gold nanorod (GNR)-AlPcS4 photosensitizer complexes.    mPEG-SH conjugated to the surface of simple polyelectrolyte-coated GNRs was verified using Fourier transform infrared spectroscopy;    this improved stability, reduced cytotoxicity, and increased the encapsulation and loading efficiency of the nanoparticle dispersions.    The GNR-photosensitizer complexes were exposed to the high/low PDT mode (high light dose = 80 mW/cm(2) for 0.5 min;    low light dose = 25 mW/cm(2) for 1.5 min), and a high PDT efficacy leads to approximately 90% tumor cell killing.    Due to synergistic plasmonic photothermal properties of the complexes, the high/low PDT mode demonstrated improved efficacy over using single wavelength continuous laser irradiation.    Additionally, no significant loss in viability was observed in cells exposed to free AlPcS4 photosensitizer under the same irradiation conditions.    Consequently, free AlPcS4 released from GNRs prior to cellular entry did not contribute to cytotoxicity of normal cells or impose limitations on the use of the high power density laser.    This high/low PDT mode may effectively lead to a safer and more efficient photodynamic therapy for superficial tumors. Keywords: AlPcS4 photosensitizer;    Gold nanorods;    High/low power density;    Near-infrared;    Photodynamic therapy (PDT);    Synergistic therapy. Related products Abbreviation: mPEG-SH Name: Methoxypoly(ethylene glycol) thiol For more product information, please contact us at: US Tel: 1-844-782-5734 US Tel: 1-844-QUAL-PEG CHN Tel: 400-918-9898 Email: sales@sinopeg.com
    View More
first page 1 2 3 4 5 6 7 8 9 10 last page

A total of 16 pages

Copyright © XIAMEN SINOPEG BIOTECH CO., LTD. All Rights Reserved.

Home

Products

News

contact