Welcome to visit us at SINOPEG's Booth #2351 at BIO International Convention 2022, San Diego, USA from 13th to 16th June. SINOPEG is mainly engaged in drug delivery system (DDS) and new medical materials business. SINOPEG has a high-level team with strong technical reserve to research, develop and produce high-quality polyethylene glycol, polyethylene glycol derivatives, block copolymers, anti-diabetic drug side chains, lipid excipients (ingredients for mRNA delivery system), ADC and ProTAC Linker, etc. Please contact us at sales@sinopeg.com #BIO

Welcome to visit us at SINOPEG's Booth #137 at Tides USA 2022, Boston, USA from 9th to 12th May. SINOPEG has a high-level team with strong technical reserve to research, develop and produce high-quality polyethylene glycol, polyethylene glycol derivatives, block copolymers, anti-diabetic drug side chains, lipid excipients (ingredients for mRNA delivery system), ADC and ProTAC Linker, etc. Please coutact us at sales@sinopeg.com

Welcome to visit us at SINOPEG's Booth #137 at Tides USA 2022, Boston, USA from 9th to 12th May.

Welcome to visit us at SINOPEG's Booth #2351 at 2022 BIO International Convention, San Diego, CA, the USA from 13th to 16th June. SINOPEG is a dynamic science company with professional R&D, Manufacturing and Sales capability. The company is mainly engaged in drug delivery system (DDS) and new medical materials business. Founded in 2011, SINOPEG has a high-level team with strong technical reserve to research, develop and produce high-quality polyethylene glycol, polyethylene glycol derivatives, block copolymers, anti-diabetic drug side chains, lipid excipients (ingredients for mRNA delivery system), ADC and ProTAC Linker, etc. SINOPEG has a technical team led by overseas returned doctors and foreign experts, established good cooperation relations with well-known universities and research institutes in China, such as Xiamen University, Fudan University and the Institute of Chemistry of Chinese Academy of Sciences. The company has the first-class technology development strength, provides services including the reaction, purification, separation and end product analysis and others according to customer needs. SINOPEG holds 24 IP, 19 pending IP, and 6 DMFs that meet FDA requirements. The company has ISO13485 certification. The laboratory and production workshop are designed and built in accordance with the cGMP standard of FDA. We follow the requirements of ICH-Q7A to organize production at scale, to provide high quality polyethylene glycol derivatives products and services to customers globally.

As COVID-19 continuing in 2021, it is necessary to take preventive measures, to accelerate the speed of research and development of COVID-19 vaccines and drugs, to advocate the innovation of new vaccines technologies, and to build the strength to win this epidemic. VacCon 2022, focus on technical breakthrough of the novel vaccines in post COVID-19 era. It will invite 60+ government regulatory experts, scientists and leading entrepreneurs who specialize in vaccine/ neutralizing antibody/ small molecule novel coronavirus drugs. The theme is "Focusing on technical breakthrough of the novel vaccines in post COVID-19 era", which will discuss the latest clinical development, before project approval and R&D in COVID-19 vaccine and drugs, and the leading practice of innovation and process development of mRNA/recombinant protein/adenovirus vector vaccine under different technical paths. SINOPEG, as one of the few companies in China to achieve the commercial production of lipid nanoparticles delivery materials, also attracted a lot of friends to communicate with us at the booth.

LNP delivery system The major COVID-19 vaccine technology routes currently under development worldwide include inactivated vaccines, mRNA vaccines, adenovirus vaccines and recombinant protein vaccines. As a new vaccine technology in the market, mRNA vaccine is also one of the most important COVID-19 vaccines in the world at present. As a new technology, why can mRNA vaccine be widely administered around the world? One important reason is that it has a very high effective protection rate. The two available mRNA vaccines have an effective protection rate of more than 90%, and BioNTech's mRNA vaccine, produced in collaboration with Pfizer, has an effective protection rate of 95%.Since vaccination began, the daily positive rate in the United States has dropped from 20 percent to 1 to 2 percent. MRNA transmits the genetic information for producing an antigen to the cells that make the protein. These cells then present the antigen to their surfaces, triggering the specific immune response needed. Eventually, when a virus invades, the immune system recognizes specific antigens and quickly and specifically attacks the virus to prevent infection. MRNA technology can not only be used as a preventive vaccine to prevent the spread of infectious diseases, but also as a therapeutic drug to treat some serious diseases, such as cancer and AIDS, due to its ability to spontaneously stimulate human immunity. MRNA has large molecular weight, strong hydrophilicity and high biological activity, but its single chain structure makes it extremely unstable and easy to be degraded, and delivery through the membrane with negative charge on the surface is also difficult. MRNA must enter the cell to encode antibodies, and the enzyme degradation and cell membrane barrier in the process of entering the cell are the biggest challenges that affect its delivery efficiency and transfection efficiency. Special modification or package delivery systems are required to achieve intracellular expression of mRNA. At present, Lipid nanoparticle (LNP) is commonly used as a carrier to deliver mRNA .Lipid nanoparticles mainly contain four components: ionizable lipids, neutral helper lipids, cholesterol, and PEGylated lipid. The neutral helper lipids are usually saturated phospholipids, which support the formation of the lamellar lipid bilayer and stabilize its structure arrangement. Cholesterol had strong membrane fusion, which promoted the intracellular uptake of mRNA and cytoplasmic entry. Pegylated lipids are located on the surface of lipid nanoparticles, improving their hydrophilicity and avoiding rapid removal by the immune system, preventing particle aggregation and increasing stability. The most critical excipients are ionizable cationic lipids, which are decisive factors in the efficiency of mRNA delivery and transfection. Mechanism of LNP mRNA delivery: before entering cells, cationic lipids can realize electrostatic complexation with negatively charged mRNA molecules to form complex...

Lipid nanoparticles (LNP) can play an important role in gene editing therapy. IntelliaTherapeutics and Regeneron recently announced that their co-development program, ntra-2001, a systemic CRISPR/Cas9 therapy, has achieved positive results in a phase I clinical trial. A single dose of NTLA2001 resulted in an average 87% decrease in serum transthyroxine protein level (TTR), with a maximum reduction of 96% at day 28.CRISPR/Cas9 is a gene-editing tool that makes permanent, precisely targeted changes to a patient's chromosome and fixes potential genetic mutations.Ntra-2001 is a CRISPR/ CAS9-based treatment for hereditary transthyroxine protein-mediated amyloidosis with polyneuropathy (ATTRV-PN). According to Intellia Therapeutics, NTRA-2001 is a targeted delivery of LNP in humans that selectively knocks out disease-causing genes and restores necessary genetic function through targeted insertion. Three of the six patients treated in the Phase I trial received a dose of 0.1mg/kg ntLA-2001 and the other three received a dose of 0.3mg/kg NTLA2001.At day 28, TTR decreased by an average of 52% in patients receiving 0.1mg/kg and 87% in patients receiving 0.3mg/kg, with a 97% reduction in one patient. As revealed in IntelliaTherapeutics' patent, LNP contains amine lipids for encapsulation and in vivo escape, neutral and helper lipids for stabilization, and cloaking lipids. In general, LNPS used on CRISPR/Cas9 include DSPC, cholesterol, PE2K-DMG and other liposomes, which are mostly similar to those used for LNPS of mRNA vaccines. XIAMEN SINOPEG BIOTECH CO., LTD. has been developing DDS sustained-release system for more than ten years, and has strong technical reserves and experienced quality team. The high-quality polyethylene glycol derivatives developed and produced by the company have been successfully applied in the long-acting modification of PEG proteins, peptides and three types of medical devices. We also supply high purity fatty acid side chain to the market for peptide modification. In recent years, SINOPEG has turned to polyethylene glycol phospholipid, polyethylene glycol block copolymer and other high-end complex preparations, and has carried out a number of projects with domestic leading pharmaceutical enterprises. Interested friends can contact us through the following ways: US Tel: 1-844-782-5734 CHN Tel: 400-918-9898 Email: sales@sinopeg.com Reference 1. Intellia Therapeutics. (2021).Source: retrieve Intellia Therapeutics: https://www.intelliatx.com/crisprcas9/types-of-edits-2/ 2.IntelliaTherapeutics(NTLA.us) and Regentium (RegN.us) announce the results of the first CRISPR clinical trial.Retrieve source: baidu: https://baijiahao.baidu.com/s?id=1703680441595581467&wfr=spider&for=pc

Despite the three-dimensional structure of tissues in vivo, the researches on the structures, functions and pathology of human tissues frequently relies on the two-dimensional (2D) model in vitro and animal model. Since the structure of monolayer in vitro model is quite different from the cell microenvironment in vivo, cell behaviors and functions, such as cell–cell interaction and cell-matrix interaction, are greatly affected. Moreover, animal model often fail to repeat the human characteristic because of species differences. Three-dimensional (3D) culture of tumor cell lines has been advocated as the alternative. It is simple and practicable and has the advantage of simulating the cell microenvironment in vivo. Matrices for 3D cell culture mimic one or more properties of the extracellular matrix (ECM) and tumor microenvironment in vivo. The 3D cell culturing matrices are generally composed of porous structures with diameter less than 300 nm, which can provide enough space for the growth of cells. The cancer cells can form 3D aggregates or spheroids inside the matrix. According to the main component, 3D cell culture matrices can be divided into two main categories: matrix based on natural materials and matrix based on synthetic materials. Matrices based on natural materials can provide a biological environment, but the mechanical performance of materials is commonly poor and the batch-to-batch discrepancy cannot be completely eliminated. Natural materials are usually used to form hydrogel composites. Synthetic scaffolds are polymers like Polyethylene glycol (PEG), Polylactide (PLA), Poly(lactide-co-glycolide) (PLGA/PLG) which are biodegradable and easy to reproduce. Among these materials, the thermogelling synthetic copolymer hydrogels with a sol-gel transition exhibit lower critical solution temperature (LCST) behavior, which is meaningful for a 3D cell culturing matrix. When the sol gel transition temperature of smart hydrogel is between 5℃ and 37℃, the matrix has advantages in further separation of materials and cell aggregates. Hydrogels have received extensive attention in tissue engineering and 3D cell culture, owing to their inherent properties such as flexible matrix, high water content, and responsive network structures. Hydrogels can be formed both chemically and physically. PEG hydrogels are excellent candidates as biomaterials because of their potential for incorporating both biophysical and biochemical cues and their prevention of non-specific protein adsorption, biocompatibility and FDA approval for use in humans. Thermo-sensitive hydrogel based on PLGA-PEG-PLGA tri-block copolymers has been used for delivery of proteins and water-insoluble drugs. The proper LCST and good biocompatibility of PLGA-PEG-PLGA tri-block copolymers make it a good choice for in vitro cell culture matrix. References 1. Caldwell A. S., Aguado B. A., Anseth K. S. Designing Microgels for Cell Culture and Controlled Assembly of Tissue Microenvironments. Adv Fu...